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  • Targeted nanopore sequencing with Cas9-guided adapter ligation
    In the present study, we describe nanopore Cas9-targeted sequencing (nCATS), an enrichment strategy that uses targeted cleavage of chromosomal DNA with Cas9 to ligate adapters for nanopore sequencing
  • Ligation sequencing gDNA - Cas9 enrichment (SQK-LSK109)
    Cas9 targeted sequencing protocol using the 'tiling' approach Targeted, amplification-free DNA sequencing using CRISPR Cas info sheet For more details about the Cas9 targeted sequencing approach, how to design probes, and general expectations and guidance, please refer to the Targeted, amplification-free DNA sequencing using CRISPR Cas info sheet
  • Targeted, amplification-free DNA sequencing using CRISPR Cas
    For Cas9 targeted sequencing, we are able to selectively protect and deprotect ends using CRISPR Cas and enrich for regions of interest in a background prior to long read nanopore sequencing of the native DNA molecule
  • Targeted nanopore sequencing with CRISPR-Cas9 | IDT
    Nanopore Cas9-Targeted Sequencing, or “nCATS,” combines nanopore sequencing technology with Cas9–guide RNA technology for targeted sequencing (Figure 1) Cas9, or CRISPR associated protein 9, is an enzyme that cuts DNA
  • Improved Cas9-targeted nanopore sequencing facilitates ultra-deep . . .
    Nanopore Cas9-targeted sequencing (nCATS) enables selective long-read sequencing of genomic regions with applications in the study of genetic disorders, cancer diagnostics, antimicrobial resistance detection, and structural variation analysis However, the method’s efficiency is limited by abundant high molecular weight genomic DNA background that persists in sequencing libraries
  • Context-Seq: CRISPR-Cas9 targeted nanopore sequencing for . . . - Nature
    Here we developed Context-Seq using Cas9 targeted sequencing paired with long-read sequencing to enrich for fragments of DNA containing two clinically relevant ARGs, blaTEM and blaCTX-M
  • Nanopore Targeted Sequencing Service | Fast, Flexible, Accurate - CD . . .
    Nanopore targeted sequencing service delivers high-coverage insights into genes, SVs, and epigenetics with Cas9, amplicon, or adaptive sampling Learn more today
  • Cas9-based enrichment for targeted long-read metabarcoding
    In this study, we have demonstrated the ability to perform targeted nanopore sequencing using Cas9-based enrichment for the identification of nematodes and microbes in metagenomics samples without PCR amplification
  • CRISPR-Cas9 Targeted Enrichment and Next-Generation Sequencing for . . .
    Similar to Nanopore Cas9-targeted sequencing (nCATS) (Figure 1C), this is a method for enriching native large fragments of the genome without the need for amplification and target denaturation, with the aim of increasing throughput, increasing enrichment specificity, reducing costs, and preserving genetic and epigenetic composition
  • Cas9 targeted nanopore sequencing with enhanced variant calling . . .
    This strategy uses targeted cleavage of DNA with Cas9, followed by selectively ligating adapters for nanopore sequencing However, ligation of nanopore adapters to random breakage points also generates a considerable number of so-called background reads, bringing the percentage of on-target reads down to merely 0 5% to 15% of the sequenced





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